Protein microarrays (also known as epitope microarrays or peptide chips) are created depending on protein relationships. A defined protein array is mounted on a carrier to detect matched unknown proteins. Antibodies, antigens, receptors, ligands, enzyme substrates, and protein binding elements may be immobilized on the carrier.
This innovative method can measure the proportional presence of hundreds of proteins in a single experiment. High-precision, high-throughput peptide array chip design and synthesis of the target protein binding site; peptide and peptide-protein responses surveillance system; antibody-specific epitope original discovery system; and an extensive epitope element-binding sample library and peptide array chip collection.
Patients’ biological samples may be analyzed using peptide microarrays to identify disease-specific markers, which can then be used to diagnose different illnesses, including cancers. On the other hand, Peptide microarray technology may be used to identify beneficial molecules from a vast number of pharmaceutical components, which is critical for the design and production of novel therapies and personalized therapy. If you are a researcher, you can buy peptides for research purposes only.
Guidelines for the use of peptide microarrays in research
An enzyme-linked immunosorbent assay (ELISA) methodology determines peptide microarray results. This chip has thousands of peptides (multiple copies) bonded to its surface. This peptide chip may be used to grow a variety of biological materials, including pure enzymes or antibodies, human or animal serum, cell lysates, and so on.
After a series of washing processes, an anti-IgG, anti-phosphotyrosine, or anti-myc secondary antibody is employed to achieve the desired specificity. In most cases, fluorescent scanners can pick up the fluorescent label on the secondary antibody. Chemiluminescence and autoradiography are two different detection techniques.
Analysis of the variations in findings of microarray screening between patient samples and healthy subject samples allows us to identify biomarkers that are significantly associated with the illness and may be used for diagnosis, monitoring, and assessment of therapeutic effects.
Peptide microarray characteristics
- Unlike standard chemical analysis techniques, Peptide detection arrays can cover a wide range of product components, which are limited to just a few peptide types per detection array.
- Produce quantitative analytical findings and evidence free of subjectivity and ambiguity, and adhere to the highest standards of scientific objectivity.
- Thousands of peptide reagents are examined simultaneously, and typical analysis techniques are incomparable in time and expense to get the same findings.
Use of microarrays of peptides
- Host humoral immune response plays a crucial function in protecting the body against infections. Epitope mapping Accurate diagnosis and vaccine development need the identification of B-cell epitopes on antigens. Li devised a procedure for analyzing sera from patients with COVID-19 (coronavirus disease 2019) using a peptide microarray constructed according to spike protein to map SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) spike linear epitopes. All characteristics of the certified peptide microarray were consistent, well-structured, and regular. This method of mapping the linear epitope of SARS-CoV-2 spikes may be used to map additional antigens using peptide microarray technology.
- Researchers have devised an easy and efficient screening approach for three-dimensional tissue microenvironment cell behavior detection using peptide microarrays. Polyethylene glycol hydrogel and peptide microarray platform were employed in the approach. Five distinct peptide motifs and 10 cell types were used to test the platform’s usefulness by researchers. Stem cells, differentiated cell lines, cancer cells, and immune cells were all represented. Different species’ cell lines are used to produce these cells. Many studies have shown that an assortment of peptide patterns and concentrations can screen for cell adhesion and morphology.
